THE HUMAN NF-KAPPA-B3 GENE ENCODING THE P65 SUBUNIT OF TRANSCRIPTION FACTOR NF-KAPPA-B IS LOCATED ON CHROMOSOME 11Q12

A YAC clone that contains the human gene NFKB3, encoding the p65 subun it of transcription factor nuclear factor kappa B (NF-kappa B), was is olated. The YAC contains the entire NFKB3 gene, which is smaller than 15 kb and present in a single copy in the genome. Fluorescence in situ hybridization with metaphase chromosomes showed two different chromos omal locations (11q12 and Xp11.4) for sequences present in the YAC. Th e NFKB3 gene was assigned to chromosome 11q12 by PCR analysis of a pan el of relevant hybrid cell lines. Thus, no linkage exists between NFKB 3 and genes encoding other known members of the NF-kappa B family. (C) 1994 Academic Press, Inc.