EFFECT OF INDUCERS ON THE ACTIVITY OF GLUTATHIONE-S-TRANSFERASE AND OTHER ENZYMES OF THE GLUTATHIONE PATHWAY IN CULTURED HUMAN KERATINOCYTES

Known inducers of the hepatic glutathione (GSH) S-transferases were te sted at the limits of their solubility as inducers of the enzyme in cu ltured human keratinocytes. Neither phenobarbital, trans-stilbene oxid e, propylthiouracil, nor butylated hydroxyanisole increased GSH S-tran sferase activity or led to the appearance of alpha- or mu-forms of the enzyme, as judged by Western blotting. Only the pi-form of the enzyme was found before and after all treatments. Thus, the enzyme is not in ducible in keratinocytes. However, 4 mM propylthiouracil did lead to a 50% increase in GSH reductase activity, and phenobarbital at 4 nM com pletely abolished GSH peroxidase and GSH reductase activity and led to a significant loss of viability.