A. Bollendorf et al., COMPARISON OF DIRECT SWIM-UP, MINI-PERCOLL, AND SEPHADEX-G10 SEPARATION PROCEDURES, Archives of andrology, 32(2), 1994, pp. 157-162
A comparison was made of three sperm separation techniques-direct swim
-up, mini-Percoll, and Sephadex G-10-on semen parameters, including co
unt, % motility, forward progression, and hypoosmotic swelling (HOS) t
est scores. Overall, the best quality sperm (less debris, best % and q
uality motility, and best HOS scores) were noted with the direct swim-
up procedure. Mini-Percoll resulted in the highest count, but the wors
t HOS score. Sephadex G-10 resulted in quality of semen specimen almos
t as good as the direct swim-up procedure, but did allow some noncellu
lar debris and bacteria to filter through the column.