COMPARISON OF DIRECT SWIM-UP, MINI-PERCOLL, AND SEPHADEX-G10 SEPARATION PROCEDURES

A comparison was made of three sperm separation techniques-direct swim -up, mini-Percoll, and Sephadex G-10-on semen parameters, including co unt, % motility, forward progression, and hypoosmotic swelling (HOS) t est scores. Overall, the best quality sperm (less debris, best % and q uality motility, and best HOS scores) were noted with the direct swim- up procedure. Mini-Percoll resulted in the highest count, but the wors t HOS score. Sephadex G-10 resulted in quality of semen specimen almos t as good as the direct swim-up procedure, but did allow some noncellu lar debris and bacteria to filter through the column.