Nm. Romero et al., EXPRESSION OF AN HETEROLOGOUS GENE ACTIVATING ACTINORHODIN BIOSYNTHESIS IN STREPTOMYCES-LIVIDANS AND STREPTOMYCES-COELICOLOR, FEMS microbiology letters, 116(3), 1994, pp. 301-306
Production of the blue-pigmented antibiotic actinorhodin resulted in a
ctivation in the non-producer strain Streptomyces lividans, but not in
the natural producer strain Streptomyces coelicolor, when transformed
with an heterologous activator gene from Streptomyces fradiae. The ge
ne encodes a 132 nucleotide-long transcript, responsible for the actin
orhodin production phenotype, and thought to act as a putative antisen
se RNA, which has been detected in the transformed S.lividans cultures
by reverse transcription followed by cyclic amplification.