EFFECTS OF MODIFYING DIETARY-PROTEIN IN THE PRESENCE AND ABSENCE OF VITAMIN-B-6, ON THE REGULATION OF PLASMA CALCIUM AND PHOSPHORUS LEVELS - POSITIVE IMPACT OF YEAST SACCHAROMYCES-CEREVISIAE

Vitamin B-6 status has been assessed according to the activation coeff icient (AC) of plasma aspartic aminotransferase (AST) activity. Fast-g rowing male one-day-old broiler chicks, divided into two groups (suppl emented with pyridoxine or not), were fed a soy protein isolate (Soyam in) or a purified amino acid diet containing or not Saccharomyces cere visiae yeast (2%), primarily used to promote the growth of the B-6-def icient animals. In one experiment, the protein level of the diet has b een increased to augment the metabolic demand for PN. The B-6-deficien t Soyamin diet B containing yeast produced a mild deficiency (without incidence of morbidity and mortality) and the deficiency was severe (a ppearance of neurological symptoms after 10 days, AC 1.5) when yeast w as deleted (diet A). The inclusion of yeast in both Soyamin and amino acid diets significantly increased plasma Ca and Pi levels (p<0.001) w hereas increasing the protein intake (diet C) had an inverse effect, r eflecting an inhibitory effect on intestinal absorption of these miner als. Changes in plasma Ca and Pi levels due to dietary treatments were summarized in terms of their theoretical ion product. The amino acid diet D produced the lowest Ca x Pi ion product and the highest value w as obtained with the yeast-containing diets B and E, regardless of the vitamin Bg intake and the dietary protein source. Plasma Ca and Pi le vels were simultaneously elevated in severe vitamin B-6 deficiency (di et A) as compared to control group (p<0.05). Although bone and blood c hemistries between control and moderately Bs-deficient groups did not differ, qualitative changes were observed in the morphology of the cor tical bone.