STATISTICAL SCANNING METHOD FOR THE OPTIMIZATION OF GRADIENT ELUTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
WANG QS GAO RY YAN BW FAN DP
Citation
Qs. Wang et al., STATISTICAL SCANNING METHOD FOR THE OPTIMIZATION OF GRADIENT ELUTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Chromatographia, 38(3-4), 1994, pp. 187-190
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
38
Issue
3-4
Year of publication
1994
Pages
187 - 190
Database
ISI
SICI code
0009-5893(1994)38:3-4<187:SSMFTO>2.0.ZU;2-A
Abstract
A computer-assisted method is presented for the optimization of separa tion in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) recta ngular design. This is followed by a two-dimension computer scanning t echnique. Resolution is used as the selection criterion. Good agreemen t was obtained between predicted data and experimental results.