A 6.5-MB YEAST ARTIFICIAL CHROMOSOME CONTIG INCORPORATING 33 DNA MARKERS ON THE HUMAN X-CHROMOSOME AT XQ122

The Xq22 region of the human X chromosome contains genes for a number of inherited disorders. Sixty-nine yeast artificial chromosome clones have been isolated and assembled into a 6.5-Mb contig that contains 33 DNA markers localized to this region. This contig extends distally fr om DXS366 to beyond DXS87 and includes the genes involved in X-linked agammaglobulinemia (btk), Fabry disease (GLA), and Pelizaeus-Merzbache r disease (PLP). The order of markers in this contig is consistent wit h the known genetic and physical mapping information of Xq22. This clo ned material provides a source from which to isolate other genes locat ed in this part of the X chromosome, (C) 1994 Academic Press, Inc.