NEW APPLICATIONS FOR THE ZINC IODIDE OSMIUM-TETROXIDE TECHNIQUE

The zinc iodide-osmium tetroxide (ZIO) fixation/staining method was ap plied for neurocytological studies and also to examine several other t issue samples including epidermal Langerhans cells, blood and bone mar row cells and lymphoid tissue. Although precise specificity cannot be attributed to the staining reaction, interesting staining patterns for different cell types were observed by using one of the ZIO staining s olutions. The significance of ZIO positivity is briefly discussed.