Dt. Morishige et Jp. Thornber, IDENTIFICATION OF A NOVEL LIGHT-HARVESTING COMPLEX-II PROTEIN (LHC IIC'), Photosynthesis research, 39(1), 1994, pp. 33-38
The carotene -chlorophyll-protein, LHC IIc, is a relatively minor comp
onent of the P S II antenna. Isolated LHC IIc contains a major protein
of 28 kDa along with a 26 kDa subunit in lower abundance. Previously,
it was not known if the 26 kDa protein was closely related to the 28
kDa LHC IIc protein or if it was a comigrating LHC IIb contaminating s
ubunit. A sequence of 20 amino acid residues was obtained by direct pr
otein micro-sequencing of an internal cyanogen bromide-derived peptide
fragment of the 26 kDa protein isolated from barley. The sequence sho
ws, and antibody reactions confirm, that the 26 kDa protein is similar
but distinct from both the 28 kDa LHC IIc and LHC IIb protein sequenc
es, indicating that there remains at least one more cab gene to be ide
ntified in higher plants. Furthermore, it is difficult to interpret th
e data in any way other than that there is a novel LHC II pigment-prot
ein (LHC IIc') that co-migrates with LHC IIc.