CLONING OF CMPE, A PLASMID-BORNE CATECHOL 2,3-DIOXYGENASE-ENCODING GENE FROM THE AROMATIC-DEGRADING AND CHLOROAROMATIC-DEGRADING PSEUDOMONAS SP HV3

Authors
YRJALA K PAULIN L KILPI S ROMANTSCHUK M
Citation
K. Yrjala et al., CLONING OF CMPE, A PLASMID-BORNE CATECHOL 2,3-DIOXYGENASE-ENCODING GENE FROM THE AROMATIC-DEGRADING AND CHLOROAROMATIC-DEGRADING PSEUDOMONAS SP HV3, Gene, 138(1-2), 1994, pp. 119-121
Citations number
13
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
138
Issue
1-2
Year of publication
1994
Pages
119 - 121
Database
ISI
SICI code
0378-1119(1994)138:1-2<119:COCAPC>2.0.ZU;2-1
Abstract
Pseudomonas sp. strain HV3 degrades aromatics and chloroaromatics. It harbours a mega-plasmid, designated pSKY4, from which the gene cmpE, e ncoding a catechol 2,3-dioxygenase (C230) catalyzing the conversion of catechol to 2-hydroxymuconic semialdehyde, was cloned and sequenced. The deduced amino acid (aa) sequence shows the highest homology, 52%, to the deduced aa sequences of xylE1 and dmpB. The deduced 307-aa sequ ence of cmpE contains the extradiol ring-cleavage signature in the sam e position as other 307-aa C230-encoding genes.