MAPPING OF 386 KB OF GENOMIC DNA IN THE HUMAN DYSTROPHIN-ENCODING GENE (DYS) USING AN ORDERED PHAGE-LAMBDA SUBLIBRARY OF A YAC CLONE CONTAINING THE DYS REGION

An integrated restriction map for HindIII and EcoRI has been construct ed for 386 kb of the human dystrophin-encoding gene by partial digest mapping of 35 overlapping lambda EMBL3cosW phage clones derived from a yeast artificial chromosome containing this region. Map construction was simplified in two ways. Firstly, the sequence arrangement of lambd a EMBL3cosW is such that only map data from cloned inserts are generat ed using partial digests of h phage DNA asymmetrically labelled at the left cos end with a complementary P-32-labelled oligodeoxyribonucleot ide. Secondly, the degree of partial digestion was standardised for ea ch restriction enzyme by using ultraviolet light-induced formation of thymine dimers in the recognition sequence to partially block the clea vage reaction. The map provides the basis for work on the analysis of chromosomal rearrangements in this region which give rise to Duchenne muscular dystrophy, and for studies of chromosome structure and functi on.