COMBINED POLYMERASE CHAIN-REACTION HYBRIDIZATION MICROPLATE ASSAY USED TO DETECT BOVINE LEUKEMIA-VIRUS AND SALMONELLA

Here we describe the use of an assay that integrates the polymerase ch ain reaction (PCR) with hybridization of the amplified product for det ection in the same microwell. Traditional PCR requires transportation of the amplified product to another system for characterization of sam ples. Transportation means time-consuming manipulation and risk of con taminating the laboratory with amplified product. Integration of ampli fication and specific product detection greatly reduces sample manipul ations and the risk of contamination. We used the assay for detection of bovine leukemia virus and Salmonella. The results were identical wi th those produced by two traditional PCR methods. This assay could eas ily be adapted for other organisms, simply by using other primers and probes.