QUANTIFICATION OF SERUM 1,5-ANHYDROGLUCITOL IN UREMIC AND DIABETIC-PATIENTS BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

We developed a new method for measuring serum 1,5-anhydroglucitol (1,5 -AG), using liquid chromatography/atmospheric pressure chemical-ioniza tion mass spectrometry (LC/MS). With this method we measured serum 1,5 -AG concentrations in uremic and diabetic patients and compared these values with those determined by an enzymatic method. Serum 1,5-AG conc entrations were significantly less in the undialyzed and dialyzed urem ic patients and in diabetic patients; the values in the dialyzed patie nts decreased after hemodialysis. In uremic patients with high concent rations of serum myo-inositol, the 1,5-AG values determined by LC/MS w ere significantly lower than those determined enzymatically. There was no significant correlation between 1,5-AG and fructosamine in the ure mic patients. These results demonstrate that serum 1,5-AG cannot be us ed as an index for glycemic control in uremic patients and that the LC /MS method is indicated in uremic patients with high concentrations of myo-inositol.