2 NOVEL DEDUCED SERINE THREONINE PROTEIN-KINASES FROM SACCHAROMYCES-CEREVISIAE/

Authors
DAHLKVIST A SUNNERHAGEN P
Citation
A. Dahlkvist et P. Sunnerhagen, 2 NOVEL DEDUCED SERINE THREONINE PROTEIN-KINASES FROM SACCHAROMYCES-CEREVISIAE/, Gene, 139(1), 1994, pp. 27-33
Citations number
27
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
139
Issue
1
Year of publication
1994
Pages
27 - 33
Database
ISI
SICI code
0378-1119(1994)139:1<27:2NDSTP>2.0.ZU;2-5
Abstract
We have cloned genes for two closely related protein kinase (PK) homol ogues from Saccharomyces cerevisiae. Sequence alignment of the kinase domain with previously characterized PK reveals the highest degree of similarity with second messenger-regulated kinases. RCK1 encodes a 58- kDa protein, and is weakly expressed. RCK2 encodes a 65-kDa protein, a nd transcripts from this gene are readily detected. RCK1 has been mapp ed to the L arm of chromosome VII, and RCK2 to chromosome XII. Disrupt ion of these genes in the S. cerevisiae genome demonstrates that both genes are non-essential.