Incorporation of crayfish hepatopancreatic extract (CHE) improved the
extraction of volatile flavor compounds and increased the extractabili
ty of nonvolatile nitrogen compounds from crab hard tissue (CHT) obtai
ned by mechanical separation of composite processing by-product. Optim
um reaction conditions for hydrolysis of CHT protein were 2 hr reactio
n with 1 mt CHE per 10g CHT at 45 degrees C. Concentration of volatile
flavor compounds increased significantly in CHE-treated CHT compared
with those from untreated substrate; however, dienals and aromatic hyd
rocarbons decreased. Pyrazines, especially 2-ethyl-6-methylpyrazine an
d 2,3-dimethylpyrazine, were in markedly higher concentrations due to
CHE treatment.