C. Sanz et al., SIMULTANEOUS HPLC DETERMINATION OF 2,5-DIMETHYL-4-HYDROXY-3 (2H)-FURANONE AND RELATED FLAVOR COMPOUNDS IN STRAWBERRIES, Journal of food science, 59(1), 1994, pp. 139-141
A rapid methodology (less than 90 min) is described for the qualitativ
e and quantitative analysis of 2,5-dimethyl-4-hydroxy-3 (2H)-furanone
(furaneol), furaneol-beta-glucoside, and 2,5-dimethyl-4-methoxy-3 (2H)
-furanone (mesifurane) in strawberries. These compounds were resolved
by HPLC with a RP C18 column, using acetate buffer and methanol as bin
ary mobile phase and detection at 280 nm, and confirmed by GC-MS. The
aqueous extraction procedure gave an average recovery of these compoun
ds from strawberries > 90%, with detection limits: 0.14 furaneol, 0.05
furaneol-beta-glucoside, and 0.36 mu g/mL mesifurane.