HYDROCARBON PSEUDOSOLUBILIZING AND EMULSIFYING PROTEINS PRODUCED BY PSEUDOMONAS-CEPACIA N1

Significant specific pseudosolubilizing activity of the n-hexadecane p seudosolubilizing factor (C16PSF), n-octadecane pseudosolubilizing fac tor (C18PSF) and pristane pseudosolubilizing factor (PrisPSF) was foun d. From the partial chemical analysis of the chromatographically purif ied samples the pseudosolubilizing factors (PSFs) and n-hexadecane emu lsifying factor (HEF-C-16) were tentatively identified as glycoprotein and lipoprotein, respectively. Each of the purified PSFs showed molec ular weight 14,000 in SDS-PAGE but they had different amino acid compo sitions. The carbohydrate component of the C16PSF consists of 93% gluc ose residues. C-12-, C-13-, C-14- and C-16-are the major fatty acids c onstituting 64% of the total fatty acids of HEF-C-16.