FRACTIONATION OF IGG FRAGMENTS USING REVERSED MICELLAR EXTRACTION

Reversed micellar extraction was applied for the fractionation of IgG fragments. With isooctane solution containing 50 mM AOT, Fab, Fc and I gG were extracted to the micellar phase. Each protein had an optimum p H range in the extraction. Fab and Fc were separated from the mixture at pH 7.0, with Fab being extracted to the micellar phase and Fc remai ning in the aqueous phase. Extracted Fab fragments were recovered by b ringing them into contact with 6M guanidine/HCl followed by dilution w ith PBS. Fab and Fc fragments were separated and recovered by reversed micellar extraction from IgG lysate digested with papain. Since the p rocedure is simple and rapid compared with column chromatography, mass preparation of the fragments can be expected by this method.