CHARACTERIZATION OF A GENE COAMPLIFIED WITH KI-RAS IN Y1-MURINE ADRENAL CARCINOMA-CELLS THAT CODES FOR A PUTATIVE MEMBRANE-PROTEIN

We report the sequence and genomic organization of a gene linked to Ki -ras in the mouse and coamplified in Y1 murine adrenal carcinoma cells . The entire 4.4-kb cDNA sequence as well as promoter and splice sites for each of the three exons was determined. The gene, designated KRAG (Ki-ras-associated gene) has a CG-rich first exon and promoter region and a long 3' untranslated region and encodes 216 amino acids. The pu tative 23.9-kDa protein has four potential transmembrane hydrophobic d omains. The hydropathy plot resembles that of certain tumor-associated antigens, including CO-029 and ME491. A potential human homologue, ES T05985, was identified and provisionally mapped to human chromosome 12 , a chromosome syntenic to mouse chromosome 6, the previously mapped l ocation of KRAG. (C) 1994 Academic Press, Inc.