CONSTRUCTION OF REPRESENTATIVE NOTL LINKING LIBRARIES SPECIFIC FOR THE TOTAL HUMAN GENOME AND FOR HUMAN CHROMOSOME-3

NotI linking clones represent valuable tools for both physical and gen etic mapping. Using procedures that we have previously described, seve ral chromosome 3-specific NotI linking libraries have been constructed . Here, we describe the construction of six independent NotI linking l ibraries specific for the total human genome. These libraries were mad e using three different vectors and two combinations of restriction en zymes. Altogether, these six libraries contain more than 1 million rec ombinant phages. Considering that the human genome contains about 3000 -5000 NotI sites, it is likely that all clonable NotI sites are presen t in these libraries. Two of the six libraries were transferred into p lasmid form. At the same time, a chromosome 3-specific EcoRI-NotI libr ary (NRL1) was constructed. This library considerably increases the re presentation of cloned NotI sites in combination with previously const ructed libraries that were made using BamHI-NotI digestion. All librar ies are available on request. (C) 1994 Academic Press, Inc.