LOCALIZATION OF THE GENE (OGDH) CODING FOR THE E1K COMPONENT OF THE ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX TO CHROMOSOME-7P13-P11.2

Alpha-ketoglutarate dehydrogenase (E1k), also designated oxoglutarate dehydrogenase (OGDH; EC 1.2.4.2), is a component of the enzyme complex that catalyzes the conversion of alpha-ketogluterate to succinyl coen zyme A, a critical step in the Krebs tricarboxylic acid cycle. Deficie ncies in the activity of this enzyme complex have been observed in bra in and peripheral cells of patients with Alzheimer's disease. This fin ding led us to localize the genes for the polypeptides that compose th e alpha-ketoglutarate dehydrogenase complex (KDGHC). The E1k locus was mapped to chromosome 7p13-p11.2 using a pair of human/rodent somatic cell hybrid panels. A second related sequence, possibly a pseudogene, was identified and mapped to chromosome 10. (C) 1994 Academic Press, I nc.