K. Ensing et al., APPLICATION OF RADIORECEPTOR ASSAYS FOR SYSTEMATIC TOXICOLOGICAL ANALYSIS .2. THEORETICAL CONSIDERATIONS AND EVALUATION, Journal of pharmaceutical and biomedical analysis, 12(1), 1994, pp. 59-63
In this paper the applicability ot radioreceptor assays tor systematic
toxicological analysis will be evaluated on a theoretical basis as we
ll as on the basis of the outcomes of the analysis of a large number o
f urine samples collected after administration of a selected number of
drugs to healthy volunteers and patients. Many drugs and other substa
nces of toxicological relevance exert their action through an interact
ion with one or more receptor (sub)types. Whether the number of person
s are using particular drugs intentionally of unintentionally, radiore
ceptor assays can be a useful tool for systematic toxicological analys
is in that they can be applied to the identification ot entire pharmac
ological classes of substances as well as pharmacologically active met
abolites. In part 1 of this paper detailed procedures for radiorecepto
r assays for benzodiazepines, anticholinergics and antihistaminics hav
e Deen described in detail in order to illustrate not only the potenti
als but also the limitations of assay conditions. Fifteen drugs were a
dministered to patients and volunteers and urine samples were collecte
d and determined with the three radioreceptor assays. The results ot t
his study underline the theoretical applicability ot receptor assays i
n systematic toxicological analysis though sample pretreatment procedu
res may contribute to an improvement in sensitivity and applicability
to other biofluids.