Ca. Gaydos et al., DIAGNOSTIC UTILITY OF PCR-ENZYME IMMUNOASSAY, CULTURE, AND SEROLOGY FOR DETECTION OF CHLAMYDIA-PNEUMONIAE IN SYMPTOMATIC AND ASYMPTOMATIC PATIENTS, Journal of clinical microbiology, 32(4), 1994, pp. 903-905
To assess the utility of PCR-enzyme immunoassay (EIA) for diagnosis of
acute infection with Chlamydia pneumoniae, we compared tissue culture
, PCR-EIA, direct fluorescent-antibody (DFA) stain, and serology in st
udies with 56 patients with respiratory symptoms and 80 asymptomatic p
ersons. Thirty-five patients were positive by either culture or PCR-EI
A, and 101 were negative by both assays. Thirty specimens from symptom
atic patients and one from an asymptomatic patient were culture positi
ve; 23 of these were also PCR-EIA positive. Of the eight culture-posit
ive, PCR-EIA-negative specimens, five were DFA negative and three were
DFA positive. Four additional specimens were culture negative and PCR
-EIA positive; of these, three were DFA positive and one was DFA negat
ive. When we used culture- and/or DFA-positive results as a reference
or ''gold standard,'' the sensitivity and specificity of PCR were 76.5
and 99.0%, respectively. When we used PCR and/or DFA-positive results
as the reference, the sensitivity of culture was 87.5%. On the basis
of single acute serum specimens, only 8 of these 35 patients had diagn
ostic antibody titers. Of the asymptomatic patients, 75% had immunoglo
bulin G or immunoglobulin M antibody to C. pneumoniae; 15 (18.8%) of t
hese had antibody levels considered to be diagnostic of acute infectio
n. This multicenter study indicates that culture and/or PCR-EIA is mor
e reliable for prompt diagnosis of C. pneumoniae infection than single
-point serology alone.