INHIBITION OF PCR-BASED ASSAY FOR BORDETELLA-PERTUSSIS BY USING CALCIUM ALGINATE FIBER AND ALUMINUM SHAFT COMPONENTS OF A NASOPHARYNGEAL SWAB

Authors
WADOWSKY RM LAUS S LIBERT T STATES SJ EHRLICH GD
Citation
Rm. Wadowsky et al., INHIBITION OF PCR-BASED ASSAY FOR BORDETELLA-PERTUSSIS BY USING CALCIUM ALGINATE FIBER AND ALUMINUM SHAFT COMPONENTS OF A NASOPHARYNGEAL SWAB, Journal of clinical microbiology, 32(4), 1994, pp. 1054-1057
Citations number
14
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
32
Issue
4
Year of publication
1994
Pages
1054 - 1057
Database
ISI
SICI code
0095-1137(1994)32:4<1054:IOPAFB>2.0.ZU;2-K
Abstract
A PCR-based assay for Bordetella pertussis was inhibited by using a ca lcium alginate fiber-tipped swab with an aluminum shaft but not by usi ng a Dacron fiber-tipped swab with a plastic shaft. The calcium algina te fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas th e aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium al ginate swab for collecting specimens for testing in PCR-based assays.