SEQUENCE-ANALYSIS OF A CDNA FOR LYSYL HYDROXYLASE ISOLATED FROM HUMANSKIN FIBROBLASTS FROM A NORMAL DONOR - DIFFERENCES FROM HUMAN PLACENTAL LYSYL HYDROXYLASE CDNA

Using polymerase chain reaction, we have isolated and sequenced a 3-kb cDNA for lysyl hydroxylase (LH) from human skin fibroblasts from an n ormal donor. Apart from two polymorphic sites, no differences were obs erved between the 2184 nt coding regions of LH cDNA from fibroblasts a nd placenta. However, four differences were observed in the 3' non-cod ing regions of the two cDNAs; three were single base changes and the f ourth a deletion of a single base. The absence of the single nucleotid e in the LH cDNA from fibroblasts resulted in the loss of an HpaII sit e that is present in the placental LH cDNA; this was confirmed in HpaI I digests of fibroblast and placental LH cDNAs from the same donor. No rthern blots showed that the LH gene was strongly expressed in fibrobl asts and Placenta and, to a lesser extent, in aortal lung, vein, carti lage, and artery.