N-ACETYL CYSTEINE IS AN EARLY BUT ALSO A LATE PREVENTIVE AGENT AGAINST CARBON TETRACHLORIDE-INDUCED LIVER NECROSIS

N-Acetyl cysteine (NAC) treatment 30 min before or 6 or 10 h after car bon tetrachloride (CCl4) administration significantly prevented the li ver necrosis produced by the hepatotoxin at 24 h. NAC pretreatment was able to partially decrease the covalent binding of CCl4 reactive meta bolites at 1 and 3 h of poisoning and, to a small extent, the concentr ation, Of CCl4 reaching the liver at 3 h. NAC also diminished partiall y the CCl4-promoted increases in lipid peroxidation at 3 h, but had an enhancing effect of its own of small intensity. Results suggest that early and late protective effects of NAC might be attributable to its prior conversion to cysteine and glutathione.