A RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF A P53-RELATED SEQUENCE IN RAT (RATTUS-NORVEGICUS)

Authors
KUNIEDA T IKADAI H IMAMICHI T TOYODA Y
Citation
T. Kunieda et al., A RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF A P53-RELATED SEQUENCE IN RAT (RATTUS-NORVEGICUS), Journal of veterinary medical science, 56(1), 1994, pp. 191-193
Citations number
19
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
56
Issue
1
Year of publication
1994
Pages
191 - 193
Database
ISI
SICI code
0916-7250(1994)56:1<191:AROAPS>2.0.ZU;2-Y
Abstract
Polymorphisms of the p53 tumor-suppressor gene and its related sequenc es were investigated among inbred rat strains. In a series of Southern blot hybridizations using the human p53 cDNA probe with various restr iction endonucleases, HindIII digests and PstI digests showed interstr ain variation in the length of hybridizing fragments. Distribution of the HindIII variant fragments among 17 inbred rat strains was identica l with that of PstI variant fragments. The variant fragments were segr egated as codominant alleles in backcross progeny, and the locus for t his restriction fragment length polymorphism of the p53-related sequen ce was found to correspond to a p53-related sequence located on rat ch romosome 9.