T. Kunieda et al., A RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF A P53-RELATED SEQUENCE IN RAT (RATTUS-NORVEGICUS), Journal of veterinary medical science, 56(1), 1994, pp. 191-193
Polymorphisms of the p53 tumor-suppressor gene and its related sequenc
es were investigated among inbred rat strains. In a series of Southern
blot hybridizations using the human p53 cDNA probe with various restr
iction endonucleases, HindIII digests and PstI digests showed interstr
ain variation in the length of hybridizing fragments. Distribution of
the HindIII variant fragments among 17 inbred rat strains was identica
l with that of PstI variant fragments. The variant fragments were segr
egated as codominant alleles in backcross progeny, and the locus for t
his restriction fragment length polymorphism of the p53-related sequen
ce was found to correspond to a p53-related sequence located on rat ch
romosome 9.