THE HUMAN GENE (CSNK2A1) CODING FOR THE CASEIN KINASE-II SUBUNIT-ALPHA IS LOCATED ON CHROMOSOME-20 AND CONTAINS TANDEMLY ARRANGED ALU REPEATS

We have isolated and characterized a 18.9-kb genomic clone representin g a central portion of the human casein kinase II (CKII) subunit alpha gene (CSNK2A1). Using the whole clone as a probe, the gene was locali zed on chromosome 20p13. The clone contains eight exons whose sequence s comprise bases 102 to 824 of the coding region of the human CKII alp ha. The exon/intron splice junctions conform to the gt/ag rule. Three of the nine introns are located at positions corresponding to those in the CKII alpha gene of the nematode Caenorhabditis elegans. The intro ns contain eight complete and eight incomplete Alu repeats. Some of th e Alu sequences are arranged in tandems of two or three, which seem to originate from insertions of younger Alu sequences into the poly(A) r egion of previously integrated Alu sequences, as indicated by flanking direct repeats. (C) 1994 Academic Press, Inc.