CHARACTERIZATION OF A MICRODISSECTION LIBRARY FROM HUMAN-CHROMOSOME REGION 3P14

Structural alterations in human chromosome region 3p14-p23 resulting i n the inactivation of one or more tumor suppressor genes are thought t o play a pathogenic role in small cell lung cancer, renal cell carcino ma, and other human neoplasms. To identify putative tumor suppressor g enes, 428 recombinant clones from a microdissection library specific f or human chromosome region 3p14 were isolated and characterized. Ninet y-six of these (22.5%) were human single-copy DNA sequences, 57 of whi ch were unique sequence clones. Forty-four of these were mapped to the microdissected region using a cell hybrid mapping panel. Within this mapping panel, four probes detected two new chromosome breakpoints tha t were previously indistinguishable from the translocation breakpoint t(3;8) in 3p14.2 in hereditary renal cell carcinoma. One probe maps to the homozygously deleted region of the small cell lung cancer cell li ne U2020. In addition, microdissection clones have been shown to be su itable for isolation of yeast artificial chromosomes. (C) 1994 Academi c Press, Inc.