21 POLYMORPHIC MARKERS FROM HUMAN-CHROMOSOME-12 FOR INTEGRATION OF GENETIC AND PHYSICAL MAPS

Twenty-one physically mapped, polymorphic markers have been developed from a chromosome 12-specific cosmid library. The markers consist of C A repeat-containing sequence-tagged sites (STSs) derived from cosmid c lones mapped by fluorescence in situ hybridization (FISH). Three metho ds for determining the sequence flanking CA microsatellites were used, including one using degenerate primer sets for direct sequence analys is. Oligonucleotide primer pairs suitable for use in polymerase chain reaction (PCR) were selected from the sequences flanking the CA micros atellite and were tested for their ability to generate unique PCR prod ucts. The informativeness of these STSs as genetic markers was determi ned by typing 10 unrelated individuals who are part of the Centre d'Et ude du Polymorphisme Humaine (EPH) pedigrees. Eleven of the 21 FISH-ma pped, polymorphic STSs are heterozygous in 7 or more of the individual s tested. Since these markers are derived from physically mapped cosmi ds, genetic linkage analysis with them will facilitate the integration of the developing physical and genetic maps of chromosome 12. (C) 199 4 Academic Press, Inc.