GENE-MAPPING OF NMDA RECEPTORS AND METABOTROPIC GLUTAMATE RECEPTORS IN THE RAT (RATTUS-NORVEGICUS)

Five N-methyl-D-aspartate receptor subunit genes and six metabotropic glutamate receptor subtype genes have been assigned to particular rat chromosomes by using a rat X mouse somatic cell hybrid clone panel. N- Methyl-D-aspartate receptor subunit genes (gene symbol, GRIN) GRIN1, G RIN2A, GRIN2B, GRIN2C, and GRIN2D have been assigned to chromosomes (C hr) 3, 10, 4, 10, and 1, respectively. Metabotropic glutamate receptor subtype genes (gene symbol, GRM) GRM1, GRM2, GRM3, GRM4, GRM5, and GR M6 have been assigned to Chr 1, 8, 4, 20, 1, and 10, respectively. In addition, GRIN2A and GRM6 loci were successfully localized on Chr 10 l inkage maps by linkage analyses. The genetic distances between loci in cM (+/-SD) are as follows: GRIN2A-28.6(+/-7.0)-RR24-23.3(+/-6.4)-MYHS E, from a linkage analysis using the (SHR X WTC)F-1 X WTC cross, and 2 (+/-2.9)-GR1M64.2(+/-2.9)-MYHSE-2.1(+/-2.1)-ASGR, SHBG-27.1 (+/-6.4)-P PY, from a linkage analysis using the (ZI x TM)F-1 X 21 cross. (C) 199 4 Academic Press, Inc.