HIGH-RESOLUTION CYTOGENETIC MAP OF HUMAN-CHROMOSOME-9 - LOCALIZATION OF 203 NEW COSMID MARKERS BY DIRECT R-BANDING FLUORESCENCE IN-SITU HYBRIDIZATION

A high-resolution cytogenetic map of human chromosome 9 was constructe d with 203 newly isolated cosmid markers by direct R-banding fluoresce nce in situ hybridization. The clones were localized preferentially to R-positive bands throughout chromosome 9. Although the number of clon es was roughly proportional to the R-band size, many more clones (ca. 80) were mapped at the q34 region compared to its physical size. Based on these cytogenetic mapping data, one can establish physical contig maps with cosmids and yeast artificial chromosomes and a genetic linka ge map essential for positional cloning of responsible genes. (C) 1994 Academic Press, Inc.