THE EFFECT OF SUBSTRATE ON THE MOLECULAR-WEIGHT OF POLY-BETA-HYDROXYBUTYRATE PRODUCED BY AZOTOBACTER-VINELANDII UWD

Authors
Citation
Gq. Chen et Wj. Page, THE EFFECT OF SUBSTRATE ON THE MOLECULAR-WEIGHT OF POLY-BETA-HYDROXYBUTYRATE PRODUCED BY AZOTOBACTER-VINELANDII UWD, Biotechnology letters, 16(2), 1994, pp. 155-160
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
01415492
Volume
16
Issue
2
Year of publication
1994
Pages
155 - 160
Database
ISI
SICI code
0141-5492(1994)16:2<155:TEOSOT>2.0.ZU;2-6
Abstract
Azotobacter vinelandii UWD produced very high molecular weight (MW) (a pprox. 4 million Daltons) poly-beta-hydroxybutyrate (PHB) when grown i n 5% w/v beet molasses medium. The polymer MW decreased as the beet mo lasses concentration was increased. Similar results were obtained in e quivalent concentrations of sucrose (as raw sugar), but the polymer MW was not greater than 1.6 million. This difference was not caused by m ore severe oxygen-limitation in the beet molasses medium. It appeared that the non-sugar components of beet molasses promoted the formation of higher MW polymer. Fish peptone, a known PHB-yield-promoter in this organism, did not promote the formation of very high MW polymer.