CYTOCHEMICAL CHARACTERIZATION OF SULFOGLYCOCONJUGATES AND SIALOGLYCOCONJUGATES OF HUMAN LARYNGEAL GLANDULAR CELLS

Authors
CASTELLS MT MADRID JF AVILES M MARTINEZMENARGUEZ JA BALLESTA J
Citation
Mt. Castells et al., CYTOCHEMICAL CHARACTERIZATION OF SULFOGLYCOCONJUGATES AND SIALOGLYCOCONJUGATES OF HUMAN LARYNGEAL GLANDULAR CELLS, The Journal of histochemistry and cytochemistry, 42(4), 1994, pp. 485-496
Citations number
54
Categorie Soggetti
Cytology & Histology
Journal title
The Journal of histochemistry and cytochemistry → ACNP
ISSN journal
00221554
Volume
42
Issue
4
Year of publication
1994
Pages
485 - 496
Database
ISI
SICI code
0022-1554(1994)42:4<485:CCOSAS>2.0.ZU;2-D
Abstract
The composition and distribution of sulfo- and sialoglycoconjugates in human laryngeal glands have been investigated at light and electron m icroscopic levels by use of peroxidase-, digoxigenin-, and colloidal g old-conjugated lectins in combination with several chemical and enzyma tic deglycosylation procedures. The present study reveals a variety of terminal oligosaccharide sequences in serous and mucous glands. Serou s cells contained glycoconjugates with terminal Neu5Ac (alpha 2-3) Gal (beta 1-4) GlcNAc, Neu5Ac (alpha 2-6) Gal/GalNAc, Neu5Ac (alpha 2-3/6 ) Gal (beta 1-3 GalNAc, GlcNAc, and Gal (beta 1-4) GlcNAc sequences. S carce SO(4)Gal(beta 1-3)GalNAc terminal oligosaccharide chains mere de tected. Serous cells show wide morphological variability of secretory granules (electron lucent, electron dense, and bizonal) with different lectin affinities. Glycoconjugates in human laryngeal mucous glands c ontained a variety of terminal oligosaccharide sequences including SO( 4)Gal(beta 1-4)GlcNAc, SO(4)Gal(beta 1-3) GalNAc, SO(4)GalNAc, and Neu 5Ac(alpha 2-3)GalNAc.