EFFECT OF PASTEURELLA-HAEMOLYTICA INFECTION ON ALPHA-1-ACID GLYCOPROTEIN AND ALBUMIN CONCENTRATIONS IN SERUM AND SUBCUTANEOUS TISSUE CHAMBER FLUID OF CALVES

Thermoplastic tissue chambers mere implanted subcutaneously in the par alumbar fossae of 10 calves. Concentrations of alpha(1)-acid glycoprot ein (AAG) and albumin in serum and subcutaneous tissue chamber fluid w ere measured before and after inoculation of Pasteurella haemolytica i nto tissue chambers. Two months after implantation, serum and tissue c hamber fluid samples were collected and all tissue chambers were then inoculated with P haemolytica. Additional serum and chamber fluid samp les were collected two, four, six and 10 days after inoculation. The c oncentrations of AAG and albumin in the samples mere measured by radia l immuno-diffusion assay and the bromcresol method, respectively. P ha emolytica inoculation resulted in an increase in the serum and chamber fluid AAG concentrations and an increase in chamber fluid albumin con centrations, suggesting that the proportion of drugs bound to serum an d interstitial proteins may be affected by P haemolytica infection.