Bb. Reinhold et al., DETAILED STRUCTURAL CHARACTERIZATION OF SUCCINOGLYCAN, THE MAJOR EXOPOLYSACCHARIDE OF RHIZOBIUM-MELILOTI RM1021, Journal of bacteriology, 176(7), 1994, pp. 1997-2002
The detailed structure of the symbiotically important exopolysaccharid
e succinoglycan from Rhizobium meliloti Rm1021 was determined by mass
spectrometry with electrospray ionization and collision-induced dissoc
iation of the octameric oligosaccharide repeating unit. Previously und
etermined locations of the succinyl and acetyl modifications were dete
rmined, in respect to both the residue locations within the octamer an
d the carbon positions within the pyranose ring. Glycosidic linkages d
etermined previously by methylation analysis were also verified.