RECONSTITUTION OF GLUCOSE-UPTAKE AND PHOSPHORYLATION IN A GLUCOSE-NEGATIVE MUTANT OF ESCHERICHIA-COLI BY USING ZYMOMONAS-MOBILIS GENES ENCODING THE GLUCOSE FACILITATOR PROTEIN AND GLUCOKINASE
Jl. Snoep et al., RECONSTITUTION OF GLUCOSE-UPTAKE AND PHOSPHORYLATION IN A GLUCOSE-NEGATIVE MUTANT OF ESCHERICHIA-COLI BY USING ZYMOMONAS-MOBILIS GENES ENCODING THE GLUCOSE FACILITATOR PROTEIN AND GLUCOKINASE, Journal of bacteriology, 176(7), 1994, pp. 2133-2135
Expression of the Zymomonas mobilis glf (glucose facilitator protein)
and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negati
ve) provided a new functional pathway for glucose uptake and phosphory
lation. Both genes were essential for the restoration of growth in glu
cose minimal medium and for acid production on glucose-Macconkey agar
plates.