S. Mihok et al., ISOLATION OF TRYPANOSOMA SPP FROM WILD TSETSE-FLIES THROUGH PROCYCLICEXPANSION IN GLOSSINA-MORSITANS CENTRALIS, Acta Tropica, 56(1), 1994, pp. 25-37
Procyclic trypanosomes from wild tsetse flies were membrane-fed to Glo
ssina morsitans centralis in order to develop an optimal technique for
propagating field isolates. A 70% success rate was achieved in isolat
ing Trypanasoma simiae and a variety of genotypes of T. congolense ori
ginating from G. pallidipes, G. brevipalpis and G. swynnertoni. Parasi
tes matured into forms infective for mammals, and could be maintained
by passage of gut forms to new groups of flies. Ln experiments with la
boratory stocks, we also passaged immature gut infections of T. congol
ense and T. brucei from Various tsetse species to G. m. centralis. The
optimal technique was investigated for procyclic T. congolense throug
h addition of various compounds to goat blood using G. m. centralis an
d G. m. morsitans as recipients. From these experiments, many approach
es to procyclic expansion appeared possible. However, a simple and pra
ctical method based on the use of fresh goat blood for rapid feeding o
f G. m. centralis is recommended. Application of this technique should
aid in the resolution of questions relating to the cryptic diversity
of Nannomonas trypanosomes in diverse host and vector communities.