INTEGRATION OF PHYSICAL, GENETIC AND CYTOGENETIC MAPS OF HUMAN-CHROMOSOME-7 - ISOLATION AND ANALYSIS OF YEAST ARTIFICIAL CHROMOSOME CLONES FOR 117 MAPPED GENETIC-MARKERS

An important goal of the human genome project is to assemble fully int egrated physical, genetic and cytogenetic maps for each human chromoso me. Towards that end, we have isolated yeast artificial chromosome (YA C) clones containing 117 of the 119 genetic markers that constitute a recently constructed, detailed genetic map of human chromosome Analysi s of these clones reveals numerous examples where adjacent genetic mar kers have been physically connected, either in individual YACs or in m ulti-YAC contigs. At present, the 117 genetic markers are contained in fewer than 80 YAC contigs, with most of these contigs uniquely ordere d relative to one another based on the genetic map positions of the co rresponding markers. These YACs and YAC contigs are estimated to conta in similar to 60 - 85% of the DNA from human chromosome 7. YACs repres enting 36 genetic markers were mapped by fluorescence in situ hybridiz ation (FISH) to metaphase chromosomes, allowing assignment of these ge netic markers to cytogenetic bands along chromosome 7 and placement of the centromere within the genetic map. Together, these studies provid e genetically and cytogenetically anchored YAC clones covering the maj ority of chromosome 7 that will be useful both for the positional clon ing of genes and as a framework for assembling a complete YAC-based ph ysical map of the chromosome.