SEPARATION AND QUANTITATION OF STEVIOSIDE AND REBAUDIOSIDE-A IN PLANT-EXTRACTS BY NORMAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND THIN-LAYER CHROMATOGRAPHY - A COMPARISON

Two similar methods, normal phase high performance liquid chromatograp hy and silica gel thin-layer chromatography with densitometry, were ap plied for the separation and quantitative determination of the diterpe ne glycosides stevioside and rebaudioside A in the leaves of the plant Stevia rebaudiana Bertoni. Results were compared and found to be very close in all basic features: resolution, accuracy and reproducibility . Thin-layer chromatrography was round to be more suitable in case of phytochemical screening of large number of samples.