Inhibitory effects of a calcium preparation on the growth of food-born
e bacteria and yeast were investigated, and its application to food wa
s advanced. The calcium preparation was made from oyster shells, and t
hen treated electrically with ohmic heating. This product has been alr
eady used as a nutrient supplement. Chemical analysis showed that this
preparation contained mainly calcium and other minor elements. The mi
nimum inhibitory concentration (MIC) to bacterial growth was measured.
This preparation and its major component Ca(OH)2 showed almost the sa
me MICs in the range of 0.07 to 0.1%. Both Gram-positive and negative
bacteria were inhibited at the same concentrations. In the case of NaO
H or KOH, MICs ranged from 0.09 to 0.15% or from 0.15 to 0.24%, respec
tively. Yeasts were inhibited at the lower concentrations than bacteri
a. Viable cell number in food decreased or did not increased by additi
on of the calcium preparation. Bacillus, Escherichia, Salmonella and S
taphylococcus inoculated to food were suppressed their growth by the c
alcium preparation. The calcium preparation was more effective than Ca
(OH)2 to get the same effects. It was shown that the calcium preparati
on used as a nutritional additive could be applied to control the micr
oorganisms in food. Also, the possibility of utilizing the alkaline ra
nge to keep the quality of food was suggested.