DETECTION OF ALPHA-L-ARABINOFURANOSIDASE ACTIVITY IN ISOELECTRIC FOCUSED GELS USING 6-BROMO-2-NAPHTHYL-ALPHA-L-ARABINOFURANOSIDE

Authors
YOSHIDA S KAWABATA Y KANEKO S NAGAMOTO Y KUSAKABE I
Citation
S. Yoshida et al., DETECTION OF ALPHA-L-ARABINOFURANOSIDASE ACTIVITY IN ISOELECTRIC FOCUSED GELS USING 6-BROMO-2-NAPHTHYL-ALPHA-L-ARABINOFURANOSIDE, Bioscience, biotechnology, and biochemistry, 58(3), 1994, pp. 580-581
Citations number
6
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
3
Year of publication
1994
Pages
580 - 581
Database
ISI
SICI code
0916-8451(1994)58:3<580:DOAAII>2.0.ZU;2-7
Abstract
For the specific detection of alpha-L-arabinofuranosidase (alpha-L-AFa se) activity in isoelectric focused gels, 6-bromo-2-naphthyl-alpha-L-a rabinofuranoside (BN-alpha-L-Araf) was synthesized by the condensation of 2,3,5-tri-O-benzoyl-alpha-L-arabinofuranosyl bromide and 6-bromo-2 -naphthol. Alpha-L-AFase activity had been detected in a gel after iso electric focusing by using the synthesized BN-alpha-L-Araf as a substr ate, and the detection for the enzyme activity was more sensitive than protein detection with Coomassie Brilliant Blue R-250.