Jj. Waite et al., TIME-COURSE OF CHOLINERGIC AND MONOAMINERGIC CHANGES IN RAT-BRAIN AFTER IMMUNOLESIONING WITH 192-IGG-SAPORIN, Neuroscience letters, 169(1-2), 1994, pp. 154-158
192 IgG-saporin, an immunotoxin targeted at the low affinity NGF recep
tor, was infused into the lateral ventricle of rat brain. Three days a
nd one week post lesion, choline acetyltransferase activity was marked
ly decreased in cortex, hippocampus, olfactory bulbs, and septum (brai
n regions innervated by the cholinergic neurons of the basal forebrain
) with no change in cerebellum, striatum or pons. Measurement of monoa
mine levels revealed increases in HVA, DOPAC and dopamine, primarily i
n the olfactory bulbs at the 28-day time point only, suggesting a comp
ensation for cholinergic inactivity. High levels of basal forebrain ch
olinergic lesioning can be obtained with this immunotoxin with minimal
or no effects on monoaminergic or other cholinergic systems.