PHOTOAFFINITY CROSS-LINKING OF F(1)ATPASE FROM SPINACH-CHLOROPLASTS BY 3'-ARYLAZIDO-BETA-ALANYL-8-AZIDO ATP

Authors
SCHAFER HJ RATHGEBER G SCHUHEN A BERZBORN RJ
Citation
Hj. Schafer et al., PHOTOAFFINITY CROSS-LINKING OF F(1)ATPASE FROM SPINACH-CHLOROPLASTS BY 3'-ARYLAZIDO-BETA-ALANYL-8-AZIDO ATP, FEBS letters, 340(3), 1994, pp. 265-268
Citations number
51
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
340
Issue
3
Year of publication
1994
Pages
265 - 268
Database
ISI
SICI code
0014-5793(1994)340:3<265:PCOFFS>2.0.ZU;2-Y
Abstract
UV irradiation of the ATPase (CF1) from spinach chloroplasts in the pr esence of 3'-arylazido-beta-alanyl-8-azido ATP (8,3'-DiN(3)ATP) result s in a nucleotide-dependent inactivation of the enzyme and in a nucleo tide-dependent formation of alpha-beta cross-links. The results demons trate an interfacial localization of the nucleotide binding sites on C F1.