Rs. Patil et al., HIGHLY EFFICIENT PLANT-REGENERATION FROM MESOPHYLL PROTOPLASTS OF INDIAN FIELD CULTIVARS OF TOMATO (LYCOPERSICON-ESCULENTUM), Plant cell, tissue and organ culture, 36(2), 1994, pp. 255-258
Three Indian cultivars of L. esculentum were assessed for shoot regene
ration from protoplast-derived calli. Consistent yields of viable prot
oplasts (> 9.0 x 106 g f.wt.-1) were obtained from leaflets of 14 days
old cultured shoots. Protoplast viability (88-94%) and plating effici
ency (55-70%) were recorded for the three cultivars. Up to 71% of the
protoplast-derived tissues regenerated shoots.