TRANSGENIC WHEAT PLANTS OBTAINED WITH POLLEN-TUBE PATHWAY METHOD

Authors
ZENG JZ WANG DJ WU YQ ZHANG J ZHOU WJ ZHU XP XU NZ
Citation
Jz. Zeng et al., TRANSGENIC WHEAT PLANTS OBTAINED WITH POLLEN-TUBE PATHWAY METHOD, Science in China. Series B, Chemistry, life sciences & earth sciences, 37(3), 1994, pp. 319-325
Citations number
20
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Science in China. Series B, Chemistry, life sciences & earth sciences → ACNP
ISSN journal
1001652X
Volume
37
Issue
3
Year of publication
1994
Pages
319 - 325
Database
ISI
SICI code
1001-652X(1994)37:3<319:TWPOWP>2.0.ZU;2-V
Abstract
Beta-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been int roduced into hexaploid wheat c.v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants . All of these plants are identified by the Southern hybridization, an d the GUS activity can be detected by using fluorogenic assay and hist ochemical stain.