MEASUREMENT OF 4-BOND H(N)-H-ALPHA J-COUPLINGS IN STAPHYLOCOCCAL NUCLEASE

Authors
VUISTER GW BAX A
Citation
Gw. Vuister et A. Bax, MEASUREMENT OF 4-BOND H(N)-H-ALPHA J-COUPLINGS IN STAPHYLOCOCCAL NUCLEASE, Journal of biomolecular NMR, 4(2), 1994, pp. 193-200
Citations number
29
Categorie Soggetti
Biology,Spectroscopy
Journal title
Journal of biomolecular NMR → ACNP
ISSN journal
09252738
Volume
4
Issue
2
Year of publication
1994
Pages
193 - 200
Database
ISI
SICI code
0925-2738(1994)4:2<193:MO4HJI>2.0.ZU;2-S
Abstract
Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequent ial H(N)-H(alpha) J-couplings in the protein staphylococcal nuclease. Substantially negative 4J(HalphaHN) values, ranging from -0.8 to -2.3 Hz, are observed when the psi angle is near +120-degrees, and the foll owing phi angle near +60-degrees. For other conformations, the four-bo nd H(N)-H(alpha) J-couplings fall between -0.5 and +0.5 Hz.