THIN-LAYER CHROMATOGRAPHIC (TLC) DETERMINATION OF PHENYTOIN IN PHARMACEUTICAL FORMULATIONS AND IDENTIFICATION OF ITS HYDROXYLATED URINARY METABOLITES

Authors
ABOULENEIN HY SERIGNESE V
Citation
Hy. Aboulenein et V. Serignese, THIN-LAYER CHROMATOGRAPHIC (TLC) DETERMINATION OF PHENYTOIN IN PHARMACEUTICAL FORMULATIONS AND IDENTIFICATION OF ITS HYDROXYLATED URINARY METABOLITES, Analytical letters, 27(4), 1994, pp. 723-729
Citations number
10
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
27
Issue
4
Year of publication
1994
Pages
723 - 729
Database
ISI
SICI code
0003-2719(1994)27:4<723:TC(DOP>2.0.ZU;2-6
Abstract
A simple quantitative TLC method is described for the determination of phenytoin (PHT) in pharmaceutical formulations (capsules and injectab les) using a scanning densitometer. The average percent recoveries for PHT capsules and injectables were found to be 98.9 +/- 0.46 and 100.5 2 +/- 0.25 respectively. The method proposed is rapid, stability-indic ating and can be adopted for routine analysis in quality control labor atories. The method was also developed for the Identification of PHT a nd its hydroxylated metabolites namely p-hydroxy phenytoin (p-HPPH) co njugated and unconjugated and the phenytoin dihydrodiol (DHD) in the u rine of epileptic adult patients.