THE ROLE OF A PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-C IN THE PRODUCTION OF DIACYLGLYCEROL FOR NITRIC-OXIDE SYNTHESIS IN MACROPHAGES ACTIVATED BY IFN-GAMMA AND LPS

Authors
SANDS WA CLARK JS LIEW FY
Citation
Wa. Sands et al., THE ROLE OF A PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-C IN THE PRODUCTION OF DIACYLGLYCEROL FOR NITRIC-OXIDE SYNTHESIS IN MACROPHAGES ACTIVATED BY IFN-GAMMA AND LPS, Biochemical and biophysical research communications, 199(2), 1994, pp. 461-466
Citations number
19
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
199
Issue
2
Year of publication
1994
Pages
461 - 466
Database
ISI
SICI code
0006-291X(1994)199:2<461:TROAPP>2.0.ZU;2-7
Abstract
Murine macrophages activated by interferon (IFN)-gamma and bacterial l ipopolysaccharide (LPS) produce large amounts of nitric oxide (NO), wh ich is a critical mediator for a variety of biological functions. The expression of this inducible NO synthase (iNOS) involves a protein kin ase C (PKC)-dependent pathway, but the mechanism for the PKC activatio n in this system is unclear. Through analysis of diacylglycerol (DAG) synthesis and choline metabolism in activated macrophages, direct evid ence is provided that NO synthesis involves the activation of an unusu al phosphatidylcholine-specific phospholipase C (PC-PLC) and not a pho sphatidylinositol-specific phospholipase C (PI-PLC) or phospholipase D (PLD). (C) 1994 Academic Press, Inc.