S. Kuroda et al., NOVEL SUBTYPE OF HUMAN ANGIOTENSIN-II TYPE-I RECEPTOR - ANALYSIS OF SIGNAL-TRANSDUCTION MECHANISM IN TRANSFECTED CHINESE-HAMSTER OVARY CELLS, Biochemical and biophysical research communications, 199(2), 1994, pp. 475-481
We examined the intracellular signal transduction of two subtypes of h
uman angiotensin II type 1 receptor (AT(1a)R and AT(1b)R) by means of
the stable expression of each receptor cDNA in Chinese hamster ovary c
ells. Both receptors showed a rapid stimulation of phosphatidylinosito
l hydrolysis, a transient increase of intracellular Ca2+ concentration
and an inhibitory action on the forskolin-induced cyclic AMP formatio
n, Interestingly, at high AII concentrations (>1 mu M), these AT(1b)R-
mediated responses were inhibited, whereas the AT(1a)R-mediated respon
ses were not. Thus, the two At(1R)s are considered to be coupled to th
e same signal transduction cascades, but to be regulated differently o
n the post-translational level (presumably desensitization). (C) 1994
Academic Press, Inc.